Coding

Part:BBa_K812054:Design

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-20)


RFP E1010 with GoldenBrick sequences


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 13
    Illegal SpeI site found at 712
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 13
    Illegal SpeI site found at 712
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 13
    Illegal SpeI site found at 712
    Illegal AgeI site found at 572
    Illegal AgeI site found at 684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 706


Design Notes

This part has been created from E1010 by PCR amplification


Source

The part E1010 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.

References